BACKGROUND:Disturbances of beta2-adrenoceptors are discussed as a pathogenic factor in atopic diseases.

METHODS:In this study the expression and function of beta2-adrenoceptors on peripheral blood leucocytes (PBL) of seven atopic patients with seasonal rhinoconjunctivitis and their seven healthy controls was evaluated in relation to disease activity. Earlier reported data during pollen season were now compared with data obtained from the same subjects after their allergic symptoms had subsided.

RESULTS:The variables that had indicated a beta2-adrenoceptor subsensitivity in the patients during pollen season returned to control values, i.e. the reduced beta2-adrenoceptor affinity, the reduced beta2-adrenoceptor sensitivity, the reduced increase of intracellular cyclic adenosine monophosphate (cAMP) content upon stimulation with isoproterenol, and the reduced cAMP plasma concentration (values no longer significantly different from those of controls). However, the variable that had suggested an increase in activity of the cAMP degrading enzyme phosphodiesterase (PDE), i.e. the reduced basal intracellular cAMP content of the patients, remained reduced after the pollen season (4.9 +/- 1.1 pmol/10(6) cells in patients vs 8.2 +/- 0.9 pmol/10(6) cells in controls: P<0.05). There were no significant differences in beta2-adrenoceptor density between patients and controls at both investigations.

CONCLUSIONS:Atopic seasonal rhinoconjunctivitis is associated with various alterations in the PBL beta2-adrenoceptor/cAMP system that depend on disease activity. The reversible beta2-adrenoceptor subsensitivity is likely to be a consequence of the disease, whereas the irreversibly decreased basal intracellular cAMP content, suggested an elevated PDE activity, might be a basic trait of atopy.

译文

背景:讨论了β2-肾上腺素受体的紊乱是特应性疾病的致病因素。

方法:本研究中β2-肾上腺素受体的表达和功能对7例季节性鼻结膜炎的特应性患者及其7名健康对照者的外周血白细胞(PBL)进行评估,以评估其与疾病活动的关系。现在将花粉季节中较早报告的数据与相同受试者变态反应症状缓解后获得的数据进行比较。

结果:表明β2肾上腺素受体亚敏性的变量患者在花粉季节恢复到控制值,即降低的β2-肾上腺素受体亲和力,降低的β2-肾上腺素受体敏感性,异丙肾上腺素刺激后细胞内环磷酸腺苷(cAMP)含量的增加减少以及cAMP血浆浓度的降低(与对照组相比有明显的差异)。然而,在花粉季节后,提示cAMP降解酶磷酸二酯酶(PDE)活性增加(即患者基础细胞内cAMP含量降低)的变量仍降低了(4.9 /-1.1 pmol / 10(6)细胞与对照组的8.2 /-0.9 pmol / 10(6)细胞相比(P <0.05)。在两次调查中,患者和对照组之间的β2肾上腺素受体密度没有显着差异。

结论:特应性季节性鼻结膜炎与PBL beta2肾上腺素受体/ cAMP系统的各种改变有关取决于疾病的活动。可逆的β2肾上腺素受体亚敏感性可能是该疾病的结果,而基础细胞内cAMP含量不可逆地降低,提示PDE活性升高,可能是特应性的基本特征。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录