Although the importance of DNA vaccines, especially as a priming immunization has been well established in numerous HIV vaccine studies, the immunogenictiy of DNA vaccines is generally moderate. Novel adjuvant is in urgent need for improving the immunogenicity of DNA vaccine. Polysaccharide and nucleic acid fraction extracted by hot phenol method from Mycobacterium bovis bacillus Calmette-Guérin, known as BCG-PSN, is a widely used immunomodulatory product in China clinical practice. In this study, we evaluated whether the BCG-PSN could serve as a novel adjuvant of DNA vaccine to trigger better cellular and humoral immune responses against the HIV-1 Env antigen in Balb/C mouse model. The BCG-PSN was mixed with 10 μg or 100 μg of pDRVI1.0gp145 (HIV-1 CN54 gp145 gene) DNA vaccine and intramuscularly immunized two or three times. We found that BCG-PSN could significantly improve the immunogenicity of DNA vaccine when co-administered with DNA vaccine. Further, at the same vaccination schedule, BCG-PSN co-immunization with 10 μg DNA vaccine could elicit cellular and humoral immune responses which were comparable to that induced by 100 μg DNA vaccine alone. Moreover, our results demonstrate that BCG-PSN can activate TLR signaling pathways and induce Th1-type cytokines secretion. These findings suggest that BCG-PSN can serve as a novel and effective adjuvant for DNA vaccination.

译文

:尽管DNA疫苗的重要性,尤其是在许多HIV疫苗研究中已经很好地确定了启动免疫的重要性,但DNA疫苗的免疫原性通常是中等的。迫切需要新型佐剂来改善DNA疫苗的免疫原性。通过热酚法从牛分枝杆菌卡介苗中提取的多糖和核酸级分称为BCG-PSN,是中国临床实践中广泛使用的免疫调节产品。在这项研究中,我们评估了BCG-PSN是否可以充当DNA疫苗的新型佐剂,从而在Balb / C小鼠模型中引发针对HIV-1 Env抗原的更好的细胞和体液免疫反应。将BCG-PSN与10μg或100μgpDRVI1.0gp145(HIV-1 CN54 gp145基因)DNA疫苗混合,然后肌内免疫2或3次。我们发现BCG-PSN与DNA疫苗同时使用可显着提高DNA疫苗的免疫原性。此外,在相同的接种时间表下,用10μgDNA疫苗进行的BCG-PSN联合免疫可以引起与单独使用100μgDNA疫苗诱导的细胞和体液免疫反应。此外,我们的结果表明BCG-PSN可以激活TLR信号通路并诱导Th1型细胞因子分泌。这些发现表明,BCG-PSN可以作为DNA疫苗的新型有效佐剂。

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