A new immunoassay technique is described which uses totally internally reflected light to excite the fluorescence of fluorescein labeled antibody which has become bound to a hapten--protein conjugate absorbed on a quartz-plate in the antibody solution. The presence of any free hapten in solution reduces the amount of antibody free to bind to the surface and thus reduces the fluorescence signal. Measurement of the decrease of the fluorescent signal then gives a measure of the concentration of free hapten present. The technique is simple, fast and has high intrinsic sensitivity and specificity. It has been demonstrated for phenyl arsonic acid and morphine. Free morphine at a concentration of 2 X 10(-7) M is readily detected.

译文

:描述了一种新的免疫测定技术,该技术利用全内反射光激发荧光素标记的抗体的荧光,该荧光素已与吸附在抗体溶液中石英板上的半抗原-蛋白质偶联物结合。溶液中任何游离半抗原的存在都会减少自由结合至表面的抗体数量,从而降低荧光信号。然后,测量荧光信号的减少,就可以测量出存在的游离半抗原的浓度。该技术简单,快速,并且具有很高的内在敏感性和特异性。已证明可用于苯a酸和吗啡。浓度为2 X 10(-7)M的游离吗啡很容易被检测到。

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