Dengue virus (DENV) is the most prevalent arbovirus in the world, found mainly in tropical regions. As clinical manifestations present frequently as nonspecific febrile illness, laboratory diagnosis is essential to confirm DENV infections and for epidemiological studies. Recombinant envelope (E) antigens of four serotypes of DENV were used to develop an immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA). To evaluate the IgG-ELISA, a panel of serum samples that had been tested previously by a plaque reduction neutralization test (PRNT) was investigated for the presence of anti-E antibodies against the four DENV serotypes. IgG-ELISA was found to have a sensitivity (91%) and specificity (98%) at a receiver-operating characteristic (ROC) optimized cutoff and demonstrated high performance as well as good indexes. A concordance of 97% was achieved between both assays, and only 21/704 (3%) samples were not concordant. The results of the present study demonstrate a moderate correlation between neutralizing antibody titers and IgG-ELISA values. These findings indicate that the recombinant protein-based IgG-ELISA is a suitable method for routine serodiagnosis, monitoring and seroepidemiological studies of DENV infections.

译文

登革热病毒(DENV)是世界上最流行的虫媒病毒,主要发现于热带地区。由于临床表现经常以非特异性高热疾病的形式出现,因此实验室诊断对于确定DENV感染和进行流行病学研究至关重要。四种血清型DENV的重组包膜(E)抗原用于开发免疫球蛋白G酶联免疫吸附测定(IgG-ELISA)。为了评估IgG-ELISA,研究了先前通过噬斑减少中和测试(PRNT)测试过的一组血清样品中是否存在针对四种DENV血清型的抗E抗体。发现IgG-ELISA在接受者操作特征(ROC)优化的临界值上具有灵敏度(91%)和特异性(98%),并表现出较高的性能和良好的指标。两种测定之间的一致性达到97%,只有21/704(3%)个样品不一致。本研究的结果表明中和抗体滴度与IgG-ELISA值之间存在适度的相关性。这些发现表明,基于重组蛋白的IgG-ELISA是用于DENV感染的常规血清诊断,监测和血清流行病学研究的合适方法。

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