We developed a direct microtiter plate enzyme immunoassay to measure estradiol-17 beta in saliva. The assay has a commercially available monoclonal antibody, raised against estradiol-17 beta-6-carboxymethyloximebovine serum albumin, and a homologous horseradish peroxidase conjugate measured colorimetrically. The detection limit (equivalent to B0-3 SD) is 365 amol/well or 7.3 pmol/L when 50-microL samples are assayed. Cross-reactivity with estrone and estriol, testosterone, or progesterone is < 0.2%. Estradiol-17 beta was measured in daily samples over five natural menstrual cycles and eight cycles stimulated as a preliminary to in vitro fertilization, and the concentrations and fluctuations found agreed with previously published data. This method gives results in approximately 3 h and may be useful for fertility monitoring and management.

译文

我们开发了一种直接的微量滴定板酶免疫测定法,用于测量唾液中的雌二醇-17β。该测定法具有针对雌二醇-17β-6-羧甲基肟肟牛血清白蛋白的市售单克隆抗体,以及用比色法测定的同源辣根过氧化物酶结合物。当检测50微升样品时,检出限(相当于B0-3 SD)为365 amol /孔或7.3 pmol / L。与雌酮和雌三醇,睾丸激素或孕酮的交叉反应性<0.2%。在体外受精的五个自然月经周期和刺激的八个周期中,对每日样品中的雌二醇17β进行了测量,发现其浓度和波动与先前公布的数据一致。该方法可在约3小时内得出结果,可能对生育力监测和管理有用。

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