PROBLEM:To evaluate the site-specific immunoregulatory mechanisms against bacterial infection in the human fallopian tubes. METHOD OF STUDY:We investigated the effects of lipopolysaccharide (LPS) on the production of CXC chemokines by cultured oviductal epithelial cells (OEC) and oviductal stromal fibroblasts (OSF). The expression of Toll-like receptor (TLR) 4 and CD14 protein in OEC and OSF were evaluated. The phosphorylation of the inhibitor kappaB-alpha (IkappaB-alpha) protein after LPS stimulation was also examined. RESULTS:Lipopolysaccharide stimulated the secretion of granulocyte chemotactic protein-2, growth-regulated oncogene-alpha, and epithelial neutrophil activating peptide-78 by OSF, but not by OEC. The phosphorylation of the IkappaB-alpha protein was not detected in OEC after stimulation by LPS, whereas IkappaB-alpha phosphorylation was observed in OSF after stimulation by LPS. The expression of the TLR4 protein and mRNA was detected only in OSF but not in OEC. The expression of CD14 was not detected in either OEC or OSF. CONCLUSION:These results suggest that epithelial cells and fibroblasts in the human fallopian tube have evolved a unique, site-specific mechanism for recognizing Gram-negative pathogens. The lack of TLR4 in OEC may be important for avoiding a state of unnecessary inflammation that could disrupt the epithelial barrier and cause irreversible tubal scarring.

译文

问题:评估针对人类输卵管细菌感染的特定部位免疫调节机制。
研究方法:我们研究了脂多糖(LPS)对培养的输卵管上皮细胞(OEC)和输卵管间质成纤维细胞(OSF)产生CXC趋化因子的影响。评估了Toll样受体(TLR)4和CD14蛋白在OEC和OSF中的表达。还检查了LPS刺激后抑制剂kappaB-alpha(IkappaB-alpha)蛋白的磷酸化。
结果:脂多糖通过OSF刺激了粒细胞趋化蛋白2的分泌,生长调节的癌基因α和上皮中性粒细胞活化肽78的分泌,而OEC则没有。 LPS刺激后在OEC中未检测到IkappaB-α蛋白的磷酸化,而LPS刺激后在OSF中观察到了IkappaB-α磷酸化。仅在OSF中检测到TLR4蛋白和mRNA的表达,而在OEC中未检测到。在OEC或OSF中均未检测到CD14的表达。
结论:这些结果表明,人输卵管中的上皮细胞和成纤维细胞已发展出一种独特的,针对特定部位的机制来识别革兰氏阴性病原体。 OEC中TLR4的缺乏对于避免不必要的炎症状态可能很重要,这种炎症可能会破坏上皮屏障并导致不可逆的输卵管瘢痕形成。

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