Related outer membrane proteins, termed secretins, participate in the secretion of macromolecules across the outer membrane of many Gram-negative bacteria. In the pullulanase-secretion system, PulS, an outer membrane-associated lipoprotein, is required both for the integrity and the proper outer membrane localization of the PulD secretin. Here we show that the PulS-binding site is located within the C-terminal 65 residues of PulD. Addition of this domain to the filamentous phage secretin, pIV, or to the unrelated maltose-binding protein rendered both proteins dependent on PulS for stability. A chimeric protein composed of bacteriophage f1 pIV and the C-terminal domain of PuID required properly localized PulS to support phage assembly. An in vivo complex formed between the pIV-PulD65 chimera and PulS was detected by co-immunoprecipitation and by affinity chromatography.

译文

:相关的外膜蛋白,称为促胰液素,参与许多革兰氏阴性细菌的外膜大分子的分泌。在支链淀粉酶分泌系统中,PulS是一种与外膜相关的脂蛋白,是PulD促胰液素的完整性和正确的外膜定位所必需的。在这里,我们显示PulS结合位点位于PulD的C末端65个残基内。该结构域添加到丝状噬菌体分泌蛋白,pIV或无关的麦芽糖结合蛋白上,使得这两种蛋白都依赖于PulS进行稳定性。由噬菌体f1 pIV和PuID的C末端结构域组成的嵌合蛋白需要适当定位的PulS才能支持噬菌体装配。通过共免疫沉淀和亲和色谱法检测到了pIV-PulD65嵌合体和PulS之间形成的体内复合物。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录