Agents to control melanogenesis are in demand for the development of cosmetics to improve pigmentation disorders of skin and hair. In this study, we examined and evaluated the effects of flavonoids on melanogenesis in the melanogenic cells model, murine B16F10 melanoma cells. In the course of this study, we found that incubation of the cells in a medium containing 10 μM of the 4'-O-methylated flavonoids, diosmetin (4'-O-methylluteolin), acacetin (4'-O-methylapigenin) or kaempferide (4'-O-methylkaempferol), increased the melanin contents of the cells 3- to 7-fold higher than the control cells. The concentration-dependence test revealed that 20 μM acacetin showed the highest effect, up to 33-fold higher than the vehicle. On the other hand, the corresponding 4'-OH-type flavonoids, luteolin, apigenin and kaempferol, had a significantly smaller effect. Furthermore, by evaluating the melanogenic proteins, we found that the cells treated with 4'-O-methylated flavonoids showed higher tyrosinase activity, as well as upregulation of tyrosinase expression, preceded by activation of cAMP response element binding protein (CREB) and extracellular signal-regulated kinases types 1 and 2 (ERK1/2). These results indicate that the 4'-O-methyl group of flavonoids plays an important role in the induction of melanogenesis by activating its major signal transduction pathway through the upregulation of phospho-CREB in murine B16F10 melanoma cells.

译文

:需要控制黑素生成的试剂来开发化妆品,以改善皮肤和头发的色素沉着障碍。在这项研究中,我们检查和评估了类黄酮对黑色素生成细胞模型鼠B16F10黑色素瘤细胞中黑色素生成的影响。在这项研究过程中,我们发现细胞在含有10μM4'-O-甲基化类黄酮,薯os皂素(4'-O-甲基木犀草素),阿沙西汀(4'-O-甲基芹菜素)或Kaempferide(4'-O-methylkaempferol)使细胞的黑色素含量比对照细胞高3至7倍。浓度依赖性试验表明,20μM醋氨蝶呤显示出最高的作用,比赋形剂高33倍。另一方面,相应的4'-OH型类黄酮,木犀草素,芹菜素和山fer酚的作用要小得多。此外,通过评估黑色素生成蛋白,我们发现用4'-O-甲基化类黄酮处理的细胞显示出更高的酪氨酸酶活性,以及​​酪氨酸酶表达的上调,然后激活cAMP反应元件结合蛋白(CREB)和细胞外信号调节的1型和2型激酶(ERK1 / 2)。这些结果表明,类黄酮的4'-O-甲基通过激活鼠B16F10黑色素瘤细胞中的磷酸-CREB激活其主要信号转导途径,从而在诱导黑色素生成中起重要作用。

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