This study was designed to measure symptoms, IL-4, IL-5, IFN-gamma, and eosinophilic cationic protein (ECP) in nasal secretions from subjects experiencing an artificial allergy season and to look for evidence of priming. Clinically relevant allergen was administered intranasally out of season to 12 asymptomatic individuals with seasonal allergic rhinitis. These individuals were then randomized to receive allergen or saline daily for the next 7 days. Nasal secretions and scrapings of nasal epithelium were obtained at baseline (day 1), 24 hours after the initial allergen administration (day 2), and 24 hours after the last instillation of allergen or saline (day 9). Nasal symptom scores (p < 0.0002), IL-5 mRNA (p = 0.03), and ECP (p < 0.02) increased after receiving the first challenge (day 2 compared with day 1). In the six subjects randomized to receive seven sequential daily challenges with allergen, symptom scores remained elevated (p < 0.02), IL-5 protein increased (p = 0.02), and IFN-gamma (p = 0.02) levels decreased (day 9 compared with day 1). In the six subjects randomized to receive seven sequential daily challenges with placebo, symptom scores, IL-5, and IFN-gamma levels were not significantly different (day 9 compared with day 1). Compared with the findings at day 2 (n = 12), the treated subjects (n = 6) had no further increase in symptoms but did show a further increase in IL-5 (p = 0.01) and a decrease in IFN-gamma (p = 0.02) at day 9. Daily instillation of moderate doses of allergen intranasally is characterized by persistent symptoms, elevation of IL-5, and reduced levels of IFN-gamma.

译文

:本研究旨在测量经历了人工过敏季节的受试者的鼻分泌物中的症状,IL-4,IL-5,IFN-γ和嗜酸性阳离子蛋白(ECP),并寻找引发的证据。临床相关的变应原是在季节外鼻内给予12例季节性变应性鼻炎的无症状个体。然后将这些个体随机分组,在接下来的7天中每天接受过敏原或生理盐水。在基线(第1天),首次施用过敏原后24小时(第2天)和最后滴入过敏原或生理盐水后第24小时(第9天)获得鼻腔分泌物和鼻上皮的刮痕。接受首次攻击后(第2天与第1天相比),鼻症状评分(p <0.0002),IL-5 mRNA(p = 0.03)和ECP(p <0.02)升高。在随机接受每日7次连续性过敏原攻击的6名受试者中,症状评分保持升高(p <0.02),IL-5蛋白升高(p = 0.02),IFN-γ(p = 0.02)水平降低(比较第9天)与第1天)。在随机接受安慰剂的七个连续每日挑战的六名受试者中,症状评分,IL-5和IFN-γ水平无显着差异(第9天与第1天相比)。与第2天的结果(n = 12)相比,接受治疗的受试者(n = 6)的症状没有进一步增加,但确实表现出IL-5进一步增加(p = 0.01)和IFN-γ降低( p = 0.02)在第9天。鼻内每日滴入中等剂量的过敏原的特征是持续症状,IL-5升高和IFN-γ水平降低。

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