BACKGROUND:Dietary supplementation with leucine and fish oil rich in omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) has previously been shown to reduce cachexia-related outcomes in C26 tumour-bearing mice. To further explore associated processes and mechanisms we investigated changes in plasma Ca2+ levels, the involvement of parathyroid hormone related protein (PTHrP), and its possible interactions with cyclooxygenase 2 (COX-2). METHODS:CD2F1 mice were subcutaneously inoculated with C26 adenocarcinoma cells or sham treated and divided in: (1) controls, (2) tumour-bearing controls, and (3) tumour-bearing receiving experimental diets. After 20 days, body and organ masses and total plasma Ca2+ levels were determined. Furthermore, effects of DHA, EPA and leucine on production of PTHrP were studied in cultured C26 cells. RESULTS:The combination of leucine and fish oil reduced tumour-associated hypercalcemia. Plasma Ca2+ levels negatively correlated with carcass mass and multiple organ masses. DHA was able to reduce PTHrP production by C26 cells in vitro. Results indicate that this effect occurred independently of COX-2 inhibition. CONCLUSION:Our results suggest that cancer-related hypercalcemia may be ameliorated by a nutritional intervention rich in leucine and fish oil. The effect of fish oil possibly relates to a DHA-induced reduction of PTHrP excretion by the tumour.

译文

背景:以前,膳食补充富含欧米伽3脂肪酸二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)的亮氨酸和鱼油可降低C26荷瘤小鼠恶病质相关的结局。为了进一步探讨相关的过程和机制,我们研究了血浆Ca2水平的变化,甲状旁腺激素相关蛋白(PTHrP)的参与及其与环氧合酶2(COX-2)的可能相互作用。
方法:对CD2F1小鼠皮下接种C26腺癌细胞或进行假手术,分为:(1)对照组,(2)荷瘤对照组和(3)接受实验饮食的荷瘤小鼠。 20天后,确定身体和器官的质量以及血浆总Ca2水平。此外,在培养的C26细胞中研究了DHA,EPA和亮氨酸对PTHrP产生的影响。
结果:亮氨酸和鱼油的组合减少了肿瘤相关的高钙血症。血浆Ca2水平与car体质量和多个器官质量负相关。在体外,DHA能够减少C26细胞产生的PTHrP。结果表明,该效应独立于COX-2抑制而发生。
结论:我们的结果表明,可通过富含亮氨酸和鱼油的营养干预措施来改善与癌症相关的高钙血症。鱼油的作用可能与DHA诱导的肿瘤引起的PTHrP排泄减少有关。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录