ADP-ribosylation factor 5 (ARF5) is a member of the ARF gene family. The ARF proteins stimulate the in vitro ADP-ribosyltransferase activity of cholera toxin and appear to play a role in vesicular trafficking in vivo. We have mapped ARF5, one of the six known mammalian ARF genes, to a well-defined yeast artificial chromosome contig on human chromosome 7q31.3. In addition, we have isolated and sequenced an approximately 3.2-kb genomic segment that contains the entire ARF5 coding region, revealing the complete intron-exon structure of the gene. With six coding exons and five introns, the genomic structure of ARF5 is unique among the mammalian ARF genes and provides insight about the evolutionary history of this ancient gene family.

译文

ADP-核糖基化因子5 (ARF5) 是ARF基因家族的成员之一。ARF蛋白刺激霍乱毒素的体外ADP-核糖基转移酶活性,并似乎在体内囊泡运输中起作用。我们已经将ARF5 (六个已知的哺乳动物ARF基因之一) 映射到人类7q31.3染色体上定义明确的酵母人工染色体重叠群。此外,我们已经分离并测序了包含整个ARF5编码区的约3.2 kb基因组片段,揭示了该基因的完整内含子-外显子结构。具有六个编码外显子和五个内含子,ARF5的基因组结构在哺乳动物ARF基因中是独特的,并提供了有关该古老基因家族进化历史的见解。

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