The expression and localization of hepatocyte growth factor/scatter factor (HGF/SF) were examined immunohistochemically in 59 human coronary artery lesions retrieved by directional coronary atherectomy and compared with the localization of transforming growth factor beta isoforms (TGF-beta 1, -beta 2, and -beta 3). In 21 of the 59 specimens (35.6%) HGF-like immunoreactivity (HGF-IR) was revealed. The HGF immunopositivity rate of 45% (14/31) in thrombotic tissue was significantly (P < 0.05) higher than the rates of 7.3% (4/55), 7.1% (3/42), and 0% (0/14) in fibrous tissue, neointimal hyperplasia and atheromatous gruel, respectively. Immunoreactivity for HGF was much weaker than that for TGF-beta isoforms in these components except in thrombotic tissue. These cells exhibiting strong HGF-IR were inflammatory cells such as monocytes/macrophages in thrombotic tissue, in tissue lesions adjacent to a thrombus, and outside the capillary walls in a portion of the neovascularized lesions. Smooth muscle cells (SMCs) hardly demonstrated HGF-IR. In contrast, in control coronary arteries obtained at autopsy, the HGF-IR was strongly expressed in SMCs. These findings suggest that HGF produced by macrophages play a part in the process of coronary plaque formation attributable to thrombus in man.

译文

免疫组织化学方法检测了定向冠状动脉粥样斑块切除术取回的59例人类冠状动脉病变中肝细胞生长因子/分散因子(HGF / SF)的表达和定位,并与转化生长因子β同工型(TGF-beta 1,-beta 2和-beta 3)。在59个样本中的21个(35.6%)中发现了类似HGF的免疫反应性(HGF-IR)。血栓形成组织中HGF免疫阳性率为45%(14/31)显着(P <0.05)分别高于7.3%(4/55),7.1%(3/42)和0%(0/14) )分别在纤维组织,新内膜增生和粥样粥样硬化中。除了血栓形成组织外,在这些组件中,HGF的免疫反应性比TGF-β亚型的免疫反应性弱得多。这些表现出强HGF-IR的细胞是炎性细胞,例如血栓形成组织中,与血栓相邻的组织病变中,以及在部分新血管形成的病变中的毛细血管壁之外的单核细胞/巨噬细胞。平滑肌细胞(SMCs)几乎没有表现出HGF-IR。相反,在尸检时获得的对照冠状动脉中,HGF-IR在SMC中强烈表达。这些发现表明,巨噬细胞产生的HGF在可归因于人类血栓的冠状斑块形成过程中起作用。

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