The level of cyclic AMP in various fractions of rat skeletal tissue was measured after in vitro or in vivo administration of parathyroid hormone and calcitonin. Incubations of bone fractions prepared from young (5 weeks of age thyroparathyroidectomized rats revealed that both parathyroid hormone and calcitonin increased the cyclic AMP level in fractions of epiphysis, metaphysis and marrow cells. Cyclic AMP accumulation in incubated perisoteum and diaphysis were induced solely by parathyroid hormone. In in vivo experiments the cyclic AMP level in the tibia of the thyroparathyroidectomized rat was increased by infusion of either parathyroid hormone or calcitonin, and the simultaneous administration of each maximally effective dose of the two hormones exhibited an additive effect. Within 2 min, parathyroid hormone infusion caused an elevation of cyclic AMP content in periosteum and metaphysis. Rapid increase of cyclic AMP in the metaphysis was also induced by calcitonin, and the effect of the two hormones on cyclic AMP accumulation in this fraction was additive. Small but significant increase of cyclic AMP in the diaphysis was detected at 5 min after the administration of parathyroid hormone. Calcitonin infusion did not show any consistent effects on periosteum and diaphysis.

译文

:在体外或体内给予甲状旁腺激素和降钙素后,测量大鼠骨骼组织各部分中环AMP的水平。从年轻(5周龄甲状腺副甲状腺切除的大鼠)制备的骨级分的培养表明,甲状旁腺激素和降钙素均会增加骨epi,干physi端和骨髓细胞部分中的环AMP含量。孵育的骨膜和骨干中的循环AMP积累仅由甲状旁腺激素诱导在体内实验中,通过注射甲状旁腺激素或降钙素可增加甲状旁腺切除大鼠的胫骨中的环AMP含量,同时最大剂量的两种激素同时给药显示出加和作用,在2分钟内,甲状旁腺激素注入引起骨膜和干meta端的环AMP含量升高,降钙素还引起干physi端的环AMP迅速增加,这两种激素对该部分中环AMP的积累具有累加作用。在5 min时检测到骨干中的环状AMP服用甲状旁腺激素后。降钙素输注对骨膜和骨干没有显示出任何一致的影响。

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