In human cerebral cortex slices noradrenaline, isoproterenol (a beta-adrenergic agonist), dopamine, apomorphine (a dopaminergic agonist), and serotonin stimulated cyclic AMP formation: noradrenaline greater than or equal to isoproterenol greater than dopamine = apomorphine = serotonin. Clonidine (and alpha-adrenergic agonist) was ineffective in stimulating cyclic AMP formation in temporal cortex slices. The stimulatory effect of noradrenaline and isoproterenol was blocked by propranolol (a beta-adrenergic blocker) but not by phentolamine (an alpha-adrenergic blocker). Pimozide (a selective dopaminergic antagonist) inhibited the increase of cyclic AMP formation induced by dopamine or apomorphine but not that induced by noradrenaline, isoproterenol, or serotonin. Neither propranolol or phentolamine had any effect on dopamine- or serotonin-stimulated cyclic AMP formation. Chlorpromazine blocked the increase of cyclic AMP formation induced by noradrenaline, dopamine or serotonin, while cyproheptadine, a putative central serotonergic antagonist, was ineffective. These observations suggest that there may be at least two monoamine-sensitive adenylate cyclases in human cerebral cortex which have the characteristics of a beta-adrenergic and a dopaminergic receptor, respectively, and also possibly a serotonergic receptor.

译文

在人大脑皮层切片中,去甲肾上腺素,异丙肾上腺素(β-肾上腺素能激动剂),多巴胺,阿扑吗啡(多巴胺能激动剂)和5-羟色胺刺激的环状AMP形成:去甲肾上腺素大于或等于异丙肾上腺素大于多巴胺=阿扑吗啡= 5-羟色胺。可乐定(和α-肾上腺素激动剂)在刺激颞叶皮质切片中的环状AMP形成方面无效。去甲肾上腺素和异丙肾上腺素的刺激作用被普萘洛尔(一种β-肾上腺素受体阻滞剂)阻断,但未被酚妥拉明(一种α-肾上腺素能阻断剂)阻断。 Pimozide(一种选择性的多巴胺能拮抗剂)抑制多巴胺或阿扑吗啡诱导的环状AMP形成的增加,但不抑制去甲肾上腺素,异丙肾上腺素或5-羟色胺诱导的环状AMP形成的增加。普萘洛尔或酚妥拉明均未对多巴胺或5-羟色胺刺激的环状AMP的形成产生任何影响。氯丙嗪阻断了去甲肾上腺素,多巴胺或5-羟色胺诱导的环AMP形成的增加,而环丙庚定(一种假定的中枢5-羟色胺能拮抗剂)无效。这些观察结果表明,人大脑皮层中可能至少存在两种​​单胺敏感的腺苷酸环化酶,它们分别具有β-肾上腺素能和多巴胺能受体的特征,还可能具有血清素能受体的特征。

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