Chemoresistance and development of relapses are ascribable to a rare cell population of tumour mass: cancer stem cells (CSCs). Targeting CSCs could increase patients' survival rate and it is important to identify molecules that can act on the main pathways of these cells. Natural bioactive compounds, of which Manuka honey (MH) is rich, could be a good opportunity to target them. This work aims to evaluate the effect of MH on CSCs-like from human colorectal carcinoma (HCT-116 cell line) enriched through the in vitro sphere-forming assay. The results showed that MH reduced the volume of the entire culture of spheroids, affecting also their morphological parameters and induced apoptosis and ROS intracellular accumulation in CSCs-like. In addition, MH decreased the mRNA expression of one of ABC transporters (ABCG2) and affected self-renewal ability through the downregulation of the mRNA expression of one of the receptor membranes of Wnt/β-catenin pathway (Frizzled 7).

译文

:化学抵抗力和复发的发展归因于罕见的肿瘤块细胞群:癌症干细胞(CSCs)。靶向CSCs可以提高患者的存活率,因此重要的是确定可以作用于这些细胞主要途径的分子。富含麦卢卡蜂蜜(MH)的天然生物活性化合物可能是针对它们的好机会。这项工作旨在评估MH对通过体外球体形成实验富集的人大肠癌(HCT-116细胞系)的CSC样的影响。结果表明,MH减少了整个球体的体积,影响了它们的形态学参数,并诱导了CSCs样细胞的凋亡和ROS的细胞内积累。此外,MH通过下调Wnt /β-catenin途径之一受体膜的mRNA表达而降低了ABC转运蛋白之一(ABCG2)的mRNA表达,并影响了自我更新能力(Frizzled 7)。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录