Recently, D-limonene-based solvents are used as a safe alternative to xylene for histological and cytological application to dissolve paraffin. We report the case of a histopathology technician with a recalcitrant hand contact dermatitis strictly related to the use of a limonene-based solvent agent. Patch tests with SIDAPA (Italian Society of Allergological, Professional and Environmental Dermatology) standard series, limonene-based solvent used by the patient and D- and L-limonene (both oxidized and nonoxidized form) and with Giemsa and methylene blue stains were performed. Patch testing gave positive results to oxidized D- and L-limonene. The patient retired from work and promptly improved and healed the hand eczema. Subsequently, the potential occurrence of limonene oxidation products in the incriminated preparation was investigated using gas chromatography-mass spectrometry. While patch test showed positive reaction to oxidized limonene, chemical analysis failed to detect oxidized limonene in the preparations used by the patient. Considering the strict relation between the use of the preparations and the appearance of symptoms, we can assume that oxidized limonene may be produced during the handling of limonene-based products, especially in the presence of oxidants stains, frequently used in histological laboratories.

译文

:最近,D-柠檬烯基溶剂被用作组织学和细胞学上溶解石蜡的二甲苯的安全替代品。我们报告了一名组织病理学技术人员患有顽固性手接触性皮炎的案例,该案例与使用柠檬烯类溶剂试剂严格相关。使用SIDAPA(意大利过敏,专业和环境皮肤病学会)标准系列,患者使用的柠檬烯基溶剂以及D-和L-柠檬烯(氧化形式和非氧化形式)以及Giemsa和亚甲基蓝染色剂进行贴剂测试。补丁测试对氧化的D-和L-柠檬烯给出了积极的结果。患者从工作中退休,并迅速康复并治愈了手部湿疹。随后,使用气相色谱-质谱法研究了所述制品中柠檬烯氧化产物的潜在发生。虽然斑贴试验显示对氧化柠檬烯呈阳性反应,但化学分析未能检测出患者使用的制剂中氧化柠檬烯。考虑到制剂的使用与症状的出现之间的严格关系,我们可以假设氧化柠檬烯可能是在柠檬烯类产品的处理过程中产生的,特别是在存在组织学实验室经常使用的氧化剂污渍的情况下。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录