PURPOSE:The most common genitourinary malignancy in China is bladder transitional cell carcinoma (TCC). Early diagnosis of new and recurrent bladder cancers, followed by timely treatment, will help decrease mortality. There are currently no satisfactory markers for bladder cancer available in clinics. Better diagnostic methods are highly demanded. EXPERIMENTAL DESIGN:In this research, we have used comprehensive expressed sequence tag analysis, serial analysis of gene expression, and microarray analysis and quickly discovered a candidate marker, urothelial carcinoma associated 1 (UCA1). The UCA1 gene was characterized and its performance as a urine marker was analyzed by reverse transcription-PCR with urine sediments. A total of 212 individuals were included in this study, 94 having bladder cancers, 33 ureter/pelvic cancers, and 85 normal and other urinary tract disease controls. RESULTS:UCA1 was identified as a novel noncoding RNA gene dramatically up-regulated in TCC and it is the most TCC-specific gene yet identified. The full-length cDNA was 1,439 bp, and sequence analysis showed that it belonged to the human endogenous retrovirus H family. Clinical tests showed that UCA1 assay was highly specific (91.8%, 78 of 85) and very sensitive (80.9%, 76 of 94) in the diagnosis of bladder cancer and was especially valuable for superficial G2-G3 patients (sensitivity 91.1%, 41 of 45). It showed excellent differential diagnostic performance in various urinary tract diseases without TCC. CONCLUSIONS:UCA1 is a very sensitive and specific unique marker for bladder cancer. It could have important implications in postoperative noninvasive follow-up. This research also highlights a shortcut to new cancer diagnostic assays through integration of in silico isolation methods with translational clinical tests based on RNA detection protocols.

译文

目的:在中国最常见的泌尿生殖系统恶性肿瘤是膀胱移行细胞癌(TCC)。尽早诊断出新发和复发性膀胱癌,然后及时进行治疗,将有助于降低死亡率。目前,临床上尚无令人满意的膀胱癌标记物。迫切需要更好的诊断方法。
实验设计:在这项研究中,我们使用了全面的表达序列标签分析,基因表达的序列分析和微阵列分析,并迅速发现了候选标记物,尿路上皮癌相关1(UCA1)。对UCA1基因进行了表征,并通过逆转录PCR与尿沉渣一起分析了其作为尿液标记物的性能。这项研究总共包括212位个体,其中94位患有膀胱癌,33位输尿管/盆腔癌以及85位正常和其他泌尿系统疾病对照。
结果:UCA1被鉴定为TCC中上调的新型非编码RNA基因,是迄今鉴定到的最具TCC特异性的基因。全长cDNA为1439 bp,序列分析表明它属于人内源性逆转录病毒H家族。临床测试表明,UCA1检测对膀胱癌的诊断具有高度特异性(91.8%,78 of 85)和非常敏感(80.9%,76 of 94),对于浅表G2-G3患者特别有价值(敏感性91.1%,41) 45)。在没有TCC的各种泌尿系统疾病中,它表现出出色的鉴别诊断性能。
结论:UCA1是膀胱癌非常敏感和特异的独特标志物。它可能对术后无创性随访产生重要影响。这项研究还突出了通过将计算机隔离方法与基于RNA检测方案的转化临床测试相集成的新癌症诊断测定方法的捷径。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录