CONTEXT:Platonia insignis Mart. (Clusiaceae), commonly known as "bacuri," is a timber and fruit native species of the Brazilian Amazon. Some plants of the Clusiaceae family have their pharmacological properties associated with the presence of xanthone and polycyclic polyprenylated acylphloroglucinols derivatives, which have antioxidant and anticarcinogenic activities.
OBJECTIVE:The aim of this study was to assess the in vivo potential of extracts, fractions, and garcinielliptone FC isolated from of Platonia insignis seeds as a natural antioxidant.
MATERIALS AND METHODS:Male Wistar rats (250-280 g; 2 months old) were treated with Tween 80 0.05% dissolved in 0.9% saline (i.p, vehicle - control group), ethanol extract (EE), hexane extract (HE), dichloromethane fraction (DMF), ethyl acetate fraction (EAF), and garcinielliptone FC (GFC) isolated from P. insignis at doses 2 mg/kg (i.p.). All groups were observed for 24 h after the treatment. The antioxidant enzymatic activities [superoxide dismutase (SOD) and catalase (CAT)] were measured using spectrophotometric methods.
RESULTS:There were no marked alterations in SOD and CAT activities in rat hippocampus after pretreatment with EE, HE, DMF, EAF, and GFC. However, the pretreatment with GFC induced a significantly increase of 13, 17, 19, and 13% in SOD activities when compared to EE, HE, DMF, or EAF groups, respectively.
DISCUSSION AND CONCLUSION:Our findings strongly support the hypothesis that GFC isolated from P. insignis has a significant potential to be used as a natural antioxidant agent probably due to the modulation of enzymatic activity of hippocampal SOD.
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【Superoxide dismutase and catalase activities in rat hippocampus pretreated with garcinielliptone FC from Platonia insignis.].
【大鼠海马超氧化物歧化酶和过氧化氢酶活性用来自柏拉图徽章的garcinielliptone FC预处理。】
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DOI：10.3109/13880209.2011.611146文章类型：杂志文章
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背景：Platonia insignis Mart。 （Clusiaceae），俗称“ bacuri”，是巴西亚马逊河地区的一种木材和水果原生种。伞形科的一些植物具有与黄酮和具有抗氧化和抗癌活性的多环多烯丙基化酰基间苯三酚衍生物有关的药理特性。
目的：本研究的目的是评估从柏叶籽作为天然抗氧化剂分离出的提取物，级分和藤黄内酯FC的体内潜力。
材料与方法：雄性Wistar大鼠（250-280μg； 2个月大），将Tween 80 0.05％溶于0.9％生理盐水（ip，赋形剂-对照组），乙醇提取物（EE），己烷提取物（HE），从徽章假单胞菌中分离出的二氯甲烷级分（DMF），乙酸乙酯级分（EAF）和藤黄素FC（GFC）的剂量为2 mg / kg（ip）。治疗后观察所有组24 h。使用分光光度法测量抗氧化剂的酶活性[超氧化物歧化酶（SOD）和过氧化氢酶（CAT）]。
结果：EE，HE，DMF，EAF和GFC预处理后，大鼠海马的SOD和CAT活性没有明显变化。但是，与EE，HE，DMF或EAF组相比，用GFC预处理分别导致SOD活性显着增加13％，17％，19％和13％。
讨论与结论：我们的发现强有力地支持了这样的假设，即从徽章假单胞菌中分离出的GFC具有潜在的天然抗氧化剂作用，这可能是由于海马SOD的酶促活性调节所致。
目的：本研究的目的是评估从柏叶籽作为天然抗氧化剂分离出的提取物，级分和藤黄内酯FC的体内潜力。
材料与方法：雄性Wistar大鼠（250-280μg； 2个月大），将Tween 80 0.05％溶于0.9％生理盐水（ip，赋形剂-对照组），乙醇提取物（EE），己烷提取物（HE），从徽章假单胞菌中分离出的二氯甲烷级分（DMF），乙酸乙酯级分（EAF）和藤黄素FC（GFC）的剂量为2 mg / kg（ip）。治疗后观察所有组24 h。使用分光光度法测量抗氧化剂的酶活性[超氧化物歧化酶（SOD）和过氧化氢酶（CAT）]。
结果：EE，HE，DMF，EAF和GFC预处理后，大鼠海马的SOD和CAT活性没有明显变化。但是，与EE，HE，DMF或EAF组相比，用GFC预处理分别导致SOD活性显着增加13％，17％，19％和13％。
讨论与结论：我们的发现强有力地支持了这样的假设，即从徽章假单胞菌中分离出的GFC具有潜在的天然抗氧化剂作用，这可能是由于海马SOD的酶促活性调节所致。
