This study, undertaken as a component of the global Dracunculiasis Eradication Program (DEP), was designed to provide molecular tools to distinguish Dracunculus medinensis, the nematode causing human dracunculiasis, from other tissue-dwelling nematodes, including other Dracunculus species that infect humans and other animals. DNA was extracted from D. medinensis and from a closely related species that infects North American carnivores, D. insignis, so that the genes coding for the small-subunit ribosomal RNA (18S rRNA) of the parasites could be amplified, sequenced and compared. Sequences were obtained for 20 specimens of D. medinensis (from humans in Pakistan, Yemen and six African countries endemic for dracunculiasis) and three of D. insignis (from raccoons trapped in the state of Georgia in the southern U.S.A.). All of the D. medinensis 18S-rRNA sequences were found to be 1819 bases long and identical. The three D. insignis 18S-rRNA sequences were also found to be identical to each other but were 1821 bases long and differed from the D. medinensis 18S- rRNA sequence at eight positions (representing a difference of 0.44%). The 18S-rRNA coding region of a Guinea worm extracted from a dog in Ghana was indistinguishable from that of the D. medinensis isolates from human cases. These results provide the basis for the molecular differentiation of D. medinensis that will permit the DEP to determine, rapidly and accurately, whether a worm recovered from an area considered dracunculiasis-free is a specimen of D. medinensis or not.

译文

:这项研究是全球根除麦地那龙线虫计划(DEP)的一部分,旨在提供分子工具,将引起人类麦地那龙线虫的线虫Dracunculus medinensis与其他居住在组织内的线虫,包括其他感染人类和人类的线虫。其他动物。 DNA是从D. medinensis和感染北美食肉动物D. insignis的密切相关物种中提取的,因此可以扩增,测序和比较编码该寄生虫小亚基核糖体RNA(18S rRNA)的基因。获得了20份D. medinensis(来自巴基斯坦,也门和六个非洲国家的麦地那龙线虫病)的标本和3份D. insignis(来自美国南部佐治亚州被困的浣熊)的序列。发现所有的D. medinensis 18S-rRNA序列长1819个碱基且相同。还发现了三个D.signis的18S-rRNA序列彼此相同,但长度为1821个碱基,并且在八个位置与Me.ensis的18S-rRNA序列不同(差异为0.44%)。从加纳的一只狗中提取的几内亚蠕虫的18S-rRNA编码区与人类病例中的D. medinensis分离株没有区别。这些结果为D. medinensis的分子分化提供了基础,这将使DEP能够快速,准确地确定从认为没有麦地那龙线虫病的地区回收的蠕虫是否是D. medinensis的标本。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录