The fadBA operon in the fatty acid beta-oxidation pathway of P. putida KCTC1639 was blocked to induce a metabolic flux of the intermediates to the biosynthesis of medium chain-length PHA (mcl-PHA). Succinate at 150 mg l(-1) stimulated cell growth and also the biosynthesis of medium chain-length-polyhydroxyalkanoate. pH-stat fed-batch cultivation of the fadA knockout mutant P. putida KCTC1639 was carried out for 60 h, in which mcl-PHA reached 8 g l(-1) with a cell dry weight of 10.3 g l(-1).

译文

:恶臭假单胞菌KCTC1639的脂肪酸β-氧化途径中的fadBA操纵子被阻断,以诱导中间体的代谢通量,以合成中等链长的PHA(mcl-PHA)。 150 mg l(-1)的琥珀酸酯刺激细胞生长,也刺激中等链长的聚羟基链烷酸酯的生物合成。对fadA敲除突变体恶臭假单胞菌KCTC1639进行pH固定补料培养60 h,其中mcl-PHA达到8 g l(-1),细胞干重为10.3 g l(-1)。

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