Poly(1-vinyl-2-pyrrolidinone) (PVP) and copolymers of 1-vinyl-2-pyrrolidinone are insoluble in water when crosslinked but they can absorb very large amounts of water to become syringe-injectable hydrogels. Such gels have been investigated recently as potential substitutes for the vitreous humour in the eye. In this study, during the cytotoxic evaluation by sulforhodamine B colorimetric assay of variously crosslinked PVP gels, it was found that many of them showed protective/growth promoting effects on 3T3 mouse fibroblasts in static cultures, a phenomenon encountered previously only with aqueous solutions of a limited number of natural or synthetic polymers. Particularly, the gels crosslinked with diethylene glycol dimethacrylate (DEGDMA) induced a significant enhancement of cell proliferation, especially in serum-free cultures. No correlation between this effect and the essential gel properties (chemical composition, viscoelasticity and equilibrium water content) could be established. The study demonstrated that crosslinked PVP hydrogels showed a serum-like growth promoting effect on an anchorage-dependent cell line, which may be due to physical protection, inability of the insoluble gels to penetrate cell membranes, and their ability to mimic the extracellular matrix.

译文

聚(1-乙烯基-2-吡咯烷酮)(PVP)和1-乙烯基-2-吡咯烷酮的共聚物在交联时不溶于水,但它们可以吸收大量的水,成为可注射注射器的水凝胶。最近已经研究了这种凝胶作为眼睛中玻璃体液的潜在替代物。在这项研究中,在通过磺基若丹明B比色法对各种交联的PVP凝胶进行细胞毒性评估时,发现它们中的许多在静态培养物中对3T3小鼠成纤维细胞均显示出保护性/促生长作用,这种现象以前仅在A的水溶液中会遇到。数量有限的天然或合成聚合物。特别是,用二甘醇二甲基丙烯酸二甲酯(DEGDMA)交联的凝胶可显着增强细胞增殖,特别是在无血清培养物中。在这种作用与基本的凝胶性质(化学组成,粘弹性和平衡水含量)之间没有相关性。该研究表明,交联的PVP水凝胶对锚定依赖性细胞系显示出类似血清的生长促进作用,这可能是由于物理保护,不溶性凝胶无法穿透细胞膜以及它们模仿细胞外基质的能力所致。

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