This study aim to synthesize silver nanoparticles (AgNPs) using Anthemis atropatana extract and to evaluate their chemical characteristics and antimicrobial and cytotoxic effects. The biosynthesis of AgNPs is verified using UV-visible spectrum which showing maximum absorption in 430 nm wavelength. Transmission electron microscope (TEM) and scanning electron microscope (SEM) results revealed that AgNPs has a spherical shape with an average size of 38.89 nanometres. The crystalline structure of green synthesized AgNPs in optimal conditions was confirmed by XRD analysis. The pattern of XRD peaks related to face-centred cubic (fcc) (111), (200), (220), (311) and (222) observed. Also, FTIR results verified the AgNPs synthesis using plant extract. In biological tests, the MTT results indicate the dose dependence of cytotoxic effects of AgNPs on colon cancer cell lines (HT29). The AgNPs had maximum cytotoxicity on HT29 cancer cell line at 100 μg/ml concentration, which were statistically significant comparing control cells (p < .001). Moreover, real time PCR and flow cytometry results confirmed the apoptotic effects of AgNPs. According to the results, it seems that the green synthesis of AgNPs is an eco-friendly and cost effective approach. This research provides insight into the development of new anticancer and antibacterial agents.

译文

:这项研究旨在使用Anthemis atropatana提取物合成银纳米颗粒(AgNP),并评估其化学特性以及抗菌和细胞毒性作用。 AgNPs的生物合成使用紫外可见光谱进行验证,该光谱在430 nm波长处显示出最大吸收。透射电子显微镜(TEM)和扫描电子显微镜(SEM)结果表明,AgNPs具有球形形状,平均大小为38.89纳米。 XRD分析证实了在最佳条件下绿色合成AgNPs的晶体结构。 XRD峰的图案与观察到的面心立方(fcc)(111),(200),(220),(311)和(222)有关。同样,FTIR结果验证了使用植物提取物合成AgNPs的能力。在生物学测试中,MTT结果表明AgNP对结肠癌细胞系(HT29)的细胞毒性作用的剂量依赖性。 AgNPs在HT29癌细胞系中以100μg/ ml的浓度具有最大的细胞毒性,与对照细胞相比,具有统计学意义(p <0.001)。此外,实时PCR和流式细胞仪结果证实了AgNPs的凋亡作用。根据结果​​,似乎绿色合成AgNPs是一种生态友好且具有成本效益的方法。这项研究为新型抗癌和抗菌剂的开发提供了见识。

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