Liver mitochondria from the great green goby Zosterisessor ophiocephalus (Pallas) normally exhibit bioenergetic variables (membrane potential 165+/-7 mV; respiratory control ratio 6.6+/-0.4; ADP/O ratio 1.85+/-0.8; means +/- s.e.m., N=6) and activities of physiological transport systems (phosphate/proton symporter, adenine nucleotide antiporter, Ca(2+) electrophoretic uniporter) comparable with those of rat liver mitochondria. When incubated in the presence of Ca(2+) and an inducer agent such as phosphate, these mitochondria undergo a complete collapse of membrane potential accompanied by a large-amplitude swelling of the matrix, influx of sucrose from the incubation medium, release of endogenous Mg(2+) and K(+) (approximately 90% of the total) and of preaccumulated Ca(2+) and oxidation of endogenous pyridine nucleotides. All these phenomena, which are completely eliminated by cyclosporin A and inhibited with different efficacies by Mg(2+) and spermine, demonstrate that the induction of the permeability transition in this type of mitochondria has characteristics similar to those described in rat liver mitochondria. In contrast, the requirement for very high Ca(2+) concentrations (greater than 100 micromol l(-1) for the induction of the permeability transition represents a very important difference that distinguishes this phenomenon in fish and mammalian mitochondria.

译文

:绿色虾虎鱼带状疱疹(Pallas)的肝线粒体通常表现出生物能变量(膜电位165 / -7 mV;呼吸控制比6.6 /-0.4; ADP / O比1.85 /-0.8;平均值/ sem,N = 6)和与大鼠肝线粒体可比的生理转运系统(磷酸/质子同向转运蛋白,腺嘌呤核苷酸反向转运蛋白,Ca(2)电泳单向转运蛋白)的活动。当在Ca(2)和诱导剂(如磷酸盐)的存在下孵育时,这些线粒体经历膜电位的完全塌陷,伴随着基质的大幅度溶胀,蔗糖从孵育培养基中大量涌入,内源性Mg释放(2)和K()(约占总数的90%)以及预先积累的Ca(2)和内源吡啶核苷酸的氧化作用。所有这些现象,完全被环孢菌素A消除,并被Mg(2)和精胺以不同的效率抑制,证明这种类型的线粒体中通透性转变的诱导具有与大鼠肝线粒体中描述的特征相似的特征。相反,对于非常高的Ca(2)浓度(大于100 micromol l(-1))的诱导渗透性过渡的要求代表了一个非常重要的区别,该区别区分了鱼类和哺乳动物的线粒体中的这一现象。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录