The hydrolysis of monoacylglycerol and diacylglycerol by rat brain microsomes was followed by measuring the release of glycerol and monooleylglycerol from dispersions of water insoluble glyceryl esters of oleic acid. The microsomes showed three lipolytic activities. One activity, optimal at pH 4.8, catalyzed the hydrolysis of diacylglycerol but not monoacylglycerol. Two other lipolytic activities, optimal at pH 8.0-8.6, catalyzed the hydrolysis of both diacylglycerol and monoacylglycerol. The pH 8.0-8.6 activities were sensitive to heat and SH-reagents. Detergents were inhibitory in all cases. Extraction of the microsomes with KCl, KSCN, urea or Triton X-100 did not change the ratio of diacylglycerol hydrolysis at pH 4.8 and 8.0. The results of subcellular fractionation studies showed that there was no significant enrichment of the acid lipase in any fraction.

译文

:通过大鼠脑微粒体水解单酰基甘油和二酰基甘油,然后测量从水不溶性油酸甘油酯分散体中甘油和单油甘油的释放。微粒体显示出三种脂解活性。一种最适于pH 4.8的活性可催化二酰基甘油的水解,但不能催化单酰基甘油的水解。两种其他的脂解活性(最适于pH 8.0-8.6)催化了二酰基甘油和单酰基甘油的水解。 pH 8.0-8.6的活性对热和SH试剂敏感。在所有情况下,洗涤剂均具有抑制作用。用KCl,KSCN,尿素或Triton X-100提取微粒体不会改变pH 4.8和8.0时二酰基甘油水解的比例。亚细胞分级分离研究的结果表明,在任何级分中都没有明显富集酸性脂肪酶。

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