The effects of 0.5 - 5 mg/l abscisic acid [ABA], 0.5 - 10 mg/l (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol [paclobutrazol] and 0.5 - 2 mg/l alpha-cyclopropyl-alpha-(4-methoxyphenyl)-5-pyrimidinemethanol [ancymidol], 0.5 - 5 mg/l gibberellic acid [GA(3)] and 15 - 100 mg/l polyethylene glycol [PEG] 4000 supplemented in half-strength Murashige and Skoog's (MS) medium on the production of the two major protoberberine-type alkaloids (D,L-tetrahydropalmatine and D-corydaline) by the tubers of somatic embryo-derived plants of Corydalis yanhusuo were examined. Somatic embryo derived plants were also maintained for 6 months on half-strength MS medium containing 0.1 mg/l GA(3) or 0.5 mg/l paclobutrazol. The alkaloid contents were determined by high performance liquid chromatography (HPLC). The analysis revealed that the contents of D,L-tetrahydropalmatine and D-corydaline in the tubers of somatic embryo-derived plants were higher than the marketed crude drug and varied with growth regulator/PEG-4000 treatment and the age of the plant.

译文

0.5 - 5 mg/l脱落酸 [ABA],0.5-10 mg/l (2RS,3RS)-1-(4-氯苯基)-4,4-二甲基-2-(1,2,4-三唑-1-基) pentan-3-ol [多效唑] 和0.5-2 mg/l α-环丙基-α-(4-甲氧基苯基)-5-嘧啶乙醇 [ancymidol],0.5-5 mg/l赤霉素 [GA(3)] 和15-100 mg/l聚乙二醇 [PEG] 4000在半强度Murashige和Skoog's (MS) 培养基中补充生产两种主要的原小檗碱型生物碱 (D,检查了延胡索体细胞胚衍生植物块茎的L-四氢巴马汀和D-延胡索。体细胞胚衍生植物也在含有0.1 mg/l GA(3) 或0.5 mg/l多效唑的半强度MS培养基上保持6个月。确定了生物碱含量通过高效液相色谱 (HPLC)。分析表明,D,体细胞胚衍生植物块茎中的L-四氢巴马汀和D-延胡索碱高于市售粗药,并且随生长调节剂/PEG 4000处理和植物年龄而变化。

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