Given the paucity of data on the distribution of serotonin (5-HT) receptors of type 6 (5-HT(6)) in the human brain, the aim of this study was to investigate their distribution in postmortem human prefrontal cortex, striatum and hippocampus by either immunohistochemical or immunofluorescence techniques. The brain samples were obtained from 6 subjects who had died for causes not involving primarily or secondarily the CNS. The 5-HT(6) receptor distribution was explored by the [(125)I]SB-258585 binding to brain membranes followed by immunohistochemical and immunofluorescence evaluations. A specific [(125)I]SB-258585 binding was detected in all the regions under investigation, whilst the content in the hippocampus and cortex being about 10-30 times lower than in the striatum. Immunohistochemistry and double-label immunofluorescence microscopy experiments, carried out in the prefrontal cortex and hippocampus only, since data in the striatum were already published, showed the presence of 5-HT(6) receptors in both pyramidal and glial cells of prefrontal cortex, while positive cells were mainly pyramidal neurons in the hippocampus. The heterogeneous distribution of 5-HT(6) receptors provides a preliminary explanation of how they might regulate different functions in different brain areas, such as, perhaps, brain trophism in the cortex and neuronal firing in the hippocampus. This study, taking into account all the limitations due to the postmortem model used, represents the starting point to explore the 5-HT(6) receptor functionality and its sub-cellular distribution.

译文

:鉴于缺乏6型血清素(5-HT)受体(5-HT(6))在人脑中的分布的数据,本研究的目的是研究它们在死后人类前额叶皮层,纹状体中的分布免疫组织化学或免疫荧光技术检测海马和海马。脑样本是从6名因主要或次要不涉及CNS的原因死亡的受试者中获得的。通过[(125)I] SB-258585与脑膜的结合,然后进行免疫组织化学和免疫荧光评估,探索了5-HT(6)受体的分布。在所有研究区域中均检测到特定的[(125)I] SB-258585结合,而海马和皮质中的含量比纹状体低约10-30倍。仅在前额叶皮层和海马中进行的免疫组织化学和双标记免疫荧光显微镜实验,因为纹状体中的数据已经发表,显示在前额叶皮层的锥体细胞和神经胶质细胞中都存在5-HT(6)受体,而阳性细胞主要是海马中的锥体神经元。 5-HT(6)受体的异质分布为它们如何在不同的大脑区域调节不同的功能提供了初步的解释,例如,大脑皮层的营养和海马神经元的放电。这项研究,考虑到由于使用死后模型的所有限制,代表了探索5-HT(6)受体功能及其亚细胞分布的起点。

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