PURPOSE:This project explored the influence of loss of heterozygosity (LOH) and microsatellite instability (MSI) of locus D8S277 to PINX1 expression of gastric cancer in Chinese people.
METHODS:LOH and MSI of locus D8S277 in 90 paraffin-embedded gastric carcinoma specimens were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Envision immunohistochemistry was used to assess the expression of PINX1.
RESULTS:The frequency of LOH was higher in cases with lymph node metastasis than those without (18.57 vs. 0.00%, P < 0.01), and was higher in the specimens that were at TNM stage III + IV than those at stage I + II (21.43 vs. 2.94%, P < 0.01). In terms of the frequency of MSI, it was lower in cases with lymph node metastasis than those without (10.00 vs. 30.00%,P < 0.05). The positive rate of PINX1 protein was higher in samples without lymph node metastasis than those with lymph node metastasis (80.00 vs. 50.00%, P < 0.01); and was higher in the cases at TNM stage I + II than those at stage III + IV; and was lower in the cases between 40 and 60 years old than those above 60 years old (43.75 vs. 65.52%, P < 0.05).
CONCLUSION:LOH and MSI of PINX1 may play major role in tumor development and regulate it through different pathways. Because LOH plays a major role in negative expression of PINX1, it can be regarded as a sign of gastric cancer development and MSI may affect the prognosis and tumor turnover.
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【The correlation of genetic instability of PINX1 gene to clinico-pathological features of gastric cancer in the Chinese population.].
【PINX1基因的遗传不稳定性与中国人群胃癌临床病理特征的相关性。】
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DOI：10.1007/s00432-008-0471-6文章类型：杂志文章
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目的：本项目探讨了D8S277基因座杂合性丧失（LOH）和微卫星不稳定性（MSI）对中国人胃癌PINX1表达的影响。
方法：采用聚合酶链反应-单链构象多态性（PCR-SSCP）检测90例石蜡包埋的胃癌D8S277位点的LOH和MSI。使用Envision免疫组化评估PINX1的表达。
结果：淋巴结转移病例的LOH频率高于无淋巴结转移者（18.57 vs.0.00％，P <0.01），TNM III期IV的标本的LOH频率高于I II期的（21.43）比2.94％，P <0.01）。就MSI的发生频率而言，有淋巴结转移的患者的MSI发生率要低于没有淋巴结转移的患者（10.00 vs. 30.00％，P <0.05）。在无淋巴结转移的样本中，PINX1蛋白的阳性率高于有淋巴结转移的样本（80.00 vs. 50.00％，P <0.01）；在TNM第II阶段的病例要高于第III IV阶段的病例；并且在40至60岁之间的情况下比60岁以上的人群要低（43.75对65.52％，P <0.05）。
结论：PINX1的LOH和MSI可能在肿瘤发展中起主要作用，并通过不同途径对其进行调控。由于LOH在PINX1的负表达中起主要作用，因此可以认为它是胃癌发展的征兆，MSI可能会影响预后和肿瘤的发生。
方法：采用聚合酶链反应-单链构象多态性（PCR-SSCP）检测90例石蜡包埋的胃癌D8S277位点的LOH和MSI。使用Envision免疫组化评估PINX1的表达。
结果：淋巴结转移病例的LOH频率高于无淋巴结转移者（18.57 vs.0.00％，P <0.01），TNM III期IV的标本的LOH频率高于I II期的（21.43）比2.94％，P <0.01）。就MSI的发生频率而言，有淋巴结转移的患者的MSI发生率要低于没有淋巴结转移的患者（10.00 vs. 30.00％，P <0.05）。在无淋巴结转移的样本中，PINX1蛋白的阳性率高于有淋巴结转移的样本（80.00 vs. 50.00％，P <0.01）；在TNM第II阶段的病例要高于第III IV阶段的病例；并且在40至60岁之间的情况下比60岁以上的人群要低（43.75对65.52％，P <0.05）。
结论：PINX1的LOH和MSI可能在肿瘤发展中起主要作用，并通过不同途径对其进行调控。由于LOH在PINX1的负表达中起主要作用，因此可以认为它是胃癌发展的征兆，MSI可能会影响预后和肿瘤的发生。
