The complete nucleotide sequence of the chloroplast genome of potato Solanum tuberosum L. cv. Desiree was determined. The circular double-stranded DNA, which consists of 155,312 bp, contains a pair of inverted repeat regions (IRa, IRb) of 25,595 bp each. The inverted repeat regions are separated by small and large single copy regions of 18,373 and 85,749 bp, respectively. The genome contains 79 proteins, 30 tRNAs, 4 rRNAs, and unidentified genes. A comparison of chloroplast genomes of seven Solanaceae species revealed that the gene content and their relative positions of S. tuberosum are similar to the other six Solanaceae species. However, undefined open reading frames (ORFs) in LSC region were highly diverged in Solanaceae species except N. sylvestris. Detailed comparison was identified by numerous indels in the intergenic regions that were mostly located in the LSC region. Among them, a single large 241-bp deletion, was not associated with direct repeats and found in only S. tuberosum, clearly discriminates a cultivated potato from wild potato species Solanum bulbocastanum. The extent of sequence divergence may provide the basis for evaluating genetic diversity within the Solanaceae species, and will be useful to examine the evolutionary processes in potato landraces.

译文

:马铃薯马铃薯L.cv.的叶绿体基因组的完整核苷酸序列。西瑞确定了。环状双链DNA长度为155,312 bp,包含一对分别为25,595 bp的反向重复区域(IRa,IRb)。反向重复区分别被大小分别为18,373和85,749 bp的大单拷贝区域隔开。基因组包含79种蛋白质,30个tRNA,4个rRNA和未鉴定的基因。比较了7种茄科植物的叶绿体基因组,发现马铃薯的基因含量及其相对位置与其他6种茄科植物相似。然而,除樟脑猪笼草外,茄科物种在LSC区域的未定义开放阅读框(ORF)差异很大。详细的比较通过基因间区域中的大量插入缺失进行鉴定,这些插入片段大多位于LSC区域中。其中,单个241 bp的大缺失与直接重复无关联,仅在马铃薯中发现,可从野生马铃薯物种Solanum bulbocastanum中清楚地辨别出栽培马铃薯。序列差异的程度可为评估茄科物种内的遗传多样性提供基础,并将有助于研究马铃薯地方品种的进化过程。

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