Burkholderia pseudomallei is the causative agent of melioidosis and represents a potential bioterrorism threat. In the current studies we have examined gene expression in B. pseudomallei in an animal model of acute melioidosis using whole-genome microarrays. Gene expression profiles were generated by comparing transcriptional levels of B. pseudomallei-expressed genes in infected hamster organs including liver, lung, and spleen following intraperitoneal and intranasal routes of infection to those from bacteria grown in vitro. Differentially expressed genes were similar in infected livers irrespective of the route of infection. Reduced expression of a number of housekeeping genes suggested a lower bacterial growth rate during infection. Energy production during growth in vivo involved specific biochemical pathways such as isomerization of 3-phosphoglycerate, catabolism of d-glucosamine and inositol, and biosynthesis of particular amino acids. In addition, the induction of genes known to be involved in oxidative phosphorylation including ubiquinol oxidase, ferredoxin oxidoreductase, and formate dehydrogenase enzymes suggested the use of alternative pathways for energy production, while the expression of genes coding for ATP-synthase and NADH-dehydrogenase enzymes was reduced. Our studies have identified differentially expressed genes which include potential virulence genes such as those for a putative phospholipase C and a putative two-component regulatory system, and they have also provided a better understanding of bacterial metabolism in response to the host environment during acute melioidosis.

译文

假性伯克霍尔德菌(Burkholderia pseudomallei)是类鼻疮的病原体,代表潜在的生物恐怖主义威胁。在当前的研究中,我们已经使用全基因组微阵列检查了在急性类鼻oid病的动物模型中假性疟原虫中的基因表达。通过比较经腹膜内和鼻内感染途径感染的仓鼠器官(包括肝,肺和脾脏)中假苹果芽孢杆菌表达的基因的转录水平与体外培养的细菌的转录水平,从而产生基因表达谱。无论感染途径如何,差异表达的基因在受感染的肝脏中都是相似的。许多管家基因的表达降低表明感染期间细菌的生长速率较低。体内生长过程中的能量产生涉及特定的生化途径,例如3-磷酸甘油酸酯的异构化,d-葡萄糖胺和肌醇的分解代谢以及特定氨基酸的生物合成。此外,诱导诱导参与氧化磷酸化的基因包括泛醇氧化酶,铁氧还蛋白氧化还原酶和甲酸脱氢酶建议使用替代途径产生能量,而编码ATP合酶和NADH脱氢酶的基因的表达减少了。我们的研究已鉴定出差异表达的基因,其中包括潜在的毒力基因,例如推定的磷脂酶C和推定的两组分调节系统,并且它们还提供了对急性类li虫病响应宿主环境的细菌代谢的更好理解。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录